Journal: Neural Regeneration Research
Article Title: Prolonged intermittent theta burst stimulation restores the balance between A 2A R- and A 1 R-mediated adenosine signaling in the 6-hydroxidopamine model of Parkinson’s disease
doi: 10.4103/NRR.NRR-D-23-01542
Figure Lengend Snippet: Effect of prolonged iTBS on adenosine-mediated signaling in crude synaptosomal fraction of caudoputamen of 6-OHDA lesioned rats. (A) Representative support membranes showing density of 68-kDa (eN/CD73) and 37-kDa (GAPDH) bands and histograms of immunoblot analysis showing eN/CD73 protein abundance in crude synaptosomal fractions of the CPu of sham-stimulated and iTBS-stimulated animals at 1 and 3 weeks after stimulation. (B) Representative support membranes showing density of 42-kDa (ADA1) and 37-kDa (GAPDH) bands and histograms of immunoblot analysis showing ADA1 protein abundance in crude synaptosomal fractions of the CPu of sham-stimulated and iTBS-stimulated animals at 1 and 3 weeks after stimulation. (C) Representative support membranes showing density of 42-kDa (A 1 R) and 37-kDa (GAPDH) bands and histograms of immunoblot analysis showing A1R protein abundance in crude synaptosomal fractions of the CPu of sham-stimulated and iTBS-stimulated animals at 1 and 3 weeks after stimulation. (D) Representative support membranes showing density of 48-kDa (A 2A R) and 37-kDa (GAPDH) bands and histograms of immunoblot analysis showing A 2A R protein abundance in crude synaptosomal fractions of the CPu of sham-stimulated and iTBS-stimulated animals at 1 and 3 weeks after stimulation. All data are expressed as mean ± SD. Dots in the graphs represent individual values. The number at the bottom of the graphs represents the number of individual animals included in analysis. All data are expressed as percentage (%) of contralateral (left) CPu (dotted line at 100%). (E) The AMP phosphohydrolase/eN activity in the crude synaptosomal fraction of the CPu was measured in sham-stimulated and iTBS-stimulated animals at 1 and 3 weeks after stimulation. The bars represent the mean activity (expressed as nmol Pi/mg/min) ± standard error of the mean (SEM) from n = 5 determinations performed in duplicate. The number at the bottom of the graphs represents the number of individual animals included in analysis. Significance shown inside graphs: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 (unpaired Student’s t -test). (F, G) Micrographs illustrate the immunoreactivity of eN/CD73 in the CPu of sham animals in both the left and right hemispheres, as well as after 3 weeks of iTBS. (H, I) Micrographs depict immunoreactive cells for A1R in the left and right CPu of sham animals, as well as after 3 weeks of iTBS. Scale bar: 500 µm. All experiments were repeated at least twice. 6-OHDA: 6-Hydroxydopamine; A1R: adenosine A1 receptor; ADA1: adenosine deaminase 1; CPu: caudoputamen; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; iTBS: intermittent theta burst stimulation; ns: not significant.
Article Snippet: A1R , Rabbit, polyclonal , 1:1000 WB , Alomone Labs , AAR-009 , .
Techniques: Western Blot, Activity Assay
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